Approved Abstracts

Prochloraz Multigeneration experiment in F1 daphnid generation from parental daphnid pre-exposed to the fungicide.



Author(s): Salesa B; Torres-Gavilá FJ; Sancho-Aguilar E; Ferrando-Rodrigo MD;
Presenter: Beatriz Salesa Landete

Multigeneration chronic toxicity tests were carried out with Daphnia magna F1-3rd brood neonates from F0 daphnid generation pre-exposed to a sublethal concentration of 380 µg/L of the fungicide prochloraz. F1-3rd brood from F0 control daphnids were used as controls.
D. magna neonates from prochloraz pre-exposed mothers were divided in two groups: one group was exposed during 21 days to the same fungicide concentration as the F0 generation was (the group was named as TT) whereas another group was maintained for 21 days in fungicide free water (this group was named TF).
The algae Nannochloris oculata (5×105 cells/mL) was used to feed animals daily.
Survival, reproduction and growth were monitored for both F1-3rd generation groups and compared to the same parameters measured in the F1-3rd control group.
The parameters used to assess prochloraz effects on daphnid reproduction were: total average of neonates per female, average brood size, time to first reproduction, average broods per female and intrinsic rate of natural increase (r). Survival and individual growth (body length) were also determined after 21 days of experiments.
At the end of the experiment, the ratio between incubator chamber size/individual total size, and the ratio caudal spine/total length of the individual were also calculated.
Survival as well as individual growth size were not affected in any of the groups tested during the 21 days experiment when compared to control daphnids.
The evaluated reproductive parameters such as brood size, time of first reproduction or average brood per female didn’t change in any of the F1 groups (TT or TL) compared to control values (p>0.05).
On the other hand, the reproductive parameter neonates per female increased significantly (p<0.05) in the F1-TT group whereas decreased in F1-TL group compared to controls (p<0.05).
The ratio between incubator chamber size/individual total size, and the ratio caudal spine/total length of the individual, increased (p<0.05) in those F1-TT daphnids compared to control measurements, in a 20% and a 23% respectively.
Statistically differences (p<0.05) were found between both F1-3rd generation groups (TT and TL) which indicated that the previous F0 daphnid prochloraz exposure had an effect on F1 progeny. However more experiments are needed to understand the multigeneration pesticide transfer in D. magna.


Keywords: multigeneration; prochloraz; Daphnia magna

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