GENDER INFLUENCES MOLECULAR AND HISTOLOGICAL BIOMARKERS IN MATURE OYSTERS Crassostrea gasar AFTER PYRENE EXPOSURE
Author(s): ISIS MAYNA MARTINS DOS REIS; MIGUEL ANGEL SALDAñA SERRANO; JACÓ JOAQUIM MATTOS; MARÍLIA NARDELLI SIEBERT; FLAVIA LUCENA ZACCHI; CAMILA LISARB VELASQUEZ BASTOLLA; DAINA DE LIMA; BÁRBARA PACHECO HARRISON RIGHETTI; LÍGIA SAMPAIO LANZARIN; GIULIA DE SOUZA BROCARDO; VANESSA SHADECK DECONTO; CARLOS HENRIQUE ARAUJO DE MIRANDA GOMES; CLAUDIO MANOEL RODRIGUES DE MELO; Universidade Federal de Santa Catarina (UFSC);
Presenter: Miguel Angel Saldaña Serrano
Marine bivalves, such as oysters, are constantly used as sentinels for evaluating of environmental contamination due to their sessile and filtering habits. As a consequence, these animals bioconcentrate organic contaminants such as polycyclic aromatic hydrocarbons (PAHs) in their tissues. PAHs are composed of two or more benzene rings and are a class of highly distributed organic pollutants in marine environments and can affect organisms due to their carcinogenic, mutagenic and/or teratogenic characteristics. Depending on the PAHs, concentration, and period of exposure, biological damage can occur leading to irreversible physiological effects in the aquatic organisms. Among the PAHs, Pyrene (PYR) is commonly found in the marine environment and originates mainly from the incomplete combustion of fossil fuels and the use of petroleum compounds. The PYR structure shows four fused aromatic rings and its metabolism have high carcinogenic potential, can cause molecular, histological, immunotoxic effects and also compromise reproduction and induce behavioral changes when oysters are exposed to this compound. Considering that gender, or reproductive stage could affect the response of animals exposed to this compound, the aim of this study was to evaluate the molecular and histological responses in females and males of oyster Crassostrea gasar in pre-spawning stage exposed to PYR (0.25 and 0.5 μM) for 24 h. PYR concentrations were quantified in water and in female and male tissues. Transcripts of genes related to biotransformation (CYP3475C, CYP2-like, CYP2AU1, CYP356A, GSTO-like, GSTM-like, SULT-like), stress mechanisms (HSP70), and reproduction (Vitellogenin, Glycoprotein) were evaluated in gills. In addition, histological changes and localization of mRNA transcripts CYP2AU1 in gills, mantle and digestive diverticulum were evaluated. Females and males in pre-spawning stage bioconcentrated PYR in their tissues. Males were more sensitive to exposure to PYR. CYP2AU1 transcripts were induced in males (p <0.05), as well as tubular atrophy was observed only in males exposed to PYR (p <0.05). Vitelogenin transcripts were lower in males (p <0.05). Given these results, it can be proposed that CYP2AU1 gene has a good potential as biomarker of exposure to PYR in oyster Crassostrea gasar and shows that the reproductive status and gender must be considered in the interpretation of biomarker responses.
Financial Support - IMM dos Reis is also grateful to the Research and Innovation Support Foundation of the State of Santa Catarina – FAPESC (scholarship) (Notice 19/2019).
Keywords: Reproductive stage; Cytochrome P450; Bivalve
Graduada em Ciências Biológicas pela Universidade Federal de São Carlos (1985) com mestrado em Ciências (Fisiologia Geral) pela Universidade de São Paulo (1990). Realizou o doutorado no Departamento de Fisiologia Geral do IBUSP e no Departamento de Zoologia da Universidade de Leicester, Inglaterra e...Saiba mais
2000-Present Professor Aquatic Ecotoxicology, University of California, Riverside, CA USA
1995-1999 Associate Professor Pharmacology and Environmental Toxicology, University of Mississippi, MS USA
1991-1995 Assistant professor Pharmacology and Toxicology, University of Arkansas for Medi...
Gisela de Aragão Umbuzeiro is a full professor at School of Technology – UNICAMP, Brazil and an adjunct professor at Wilson College of Textiles, NCSU, USA. She graduated in Biology at University of Campinas - UNICAMP (1979), Ph.D. in Genetics also at UNICAMP (1990). Worked for 22 years at the Enviro...Saiba mais
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