Approved Abstracts

Effect of fluoxetine on Daphnia magna based on transgenerational reproduction and attraction-repellence responses

Author(s): Stemmel HL; Ramos-Rodriguez E.; Parra G.; Weiss LC; Araújo CVM;
Presenter: Helmut Stremmel

Fluoxetine an SSRI (Selective Serotonin Reuptake Inhibitor) is a common pharmaceutical used as antidepressant. As the use of fluoxetine has experienced an important increase, some European and mostly North American rivers have shown levels of fluoxetine considered potentially dangerous for biota. In our study, we aimed to show the effects of fluoxetine on the Cladocera Daphnia magna applying two different approaches: transgenerational effects on reproduction and on the avoidance-colonization behavior. In the transgenerational reproduction tests, daphnids (25 individuals per L) were exposed to two environmentally relevant concentrations of fluoxetine (control, 0.1 and 1.0 µg/L; n=5). The parental generation was exposed for 14 days, and neonates were counted on days 9 and 14. At day 14, neonates (n=25) were similarly taken from P0, F1 and F2 and transferred to a new aquarium (1 L) containing the same concentrations and maintained for 14 days. Adults (10 days old) from the last generation (F3) were tested for avoidance-colonization response in the HeMHAS (Heterogeneous Multi-Habitat Assay-System). In these experiments, a gradient of fluoxetine (0, 0.1, 0.5 and 1.0 µg/L), along which organisms were able to move freely, was created. Organisms were introduced in the control compartment and the concentration at which they colonized was recorded during 24 h. Our results indicate that there were no statistically significant differences in the reproduction of daphnids among the three treatments along the three generations. Moreover, Daphnia populations from the three treatments were able to colonize the environments with fluoxetine in the same way. To simulate a worse scenario of fluoxetine pollution, we tested organisms for reproduction effect (21-day exposure) and avoidance response using a range from 5 to 800 µg/L, above the environmentally relevant concentrations. Finally, our study brings a discussion about the risk that fluoxetine can produce to aquatic ecosystems if its indiscriminate use increases.

Keywords: HeMHAS; Pharmaceutical; Behaviour




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