Approved Abstracts

Fish acute toxicity test and nanomaterials: monitoring exposure to increase comparability among studies



Author(s): Pulido-Reyes G; Moreno-Martín G; Gómez-Gómez B; Navas JM; Madrid Y; Fernández-Cruz ML;
Presenter: María Luisa Fernández-Cruz

Fish acute toxicity tests is one of the most commonly used aquatic ecotoxicity tests, being also required in international chemical regulations. A general trend in the toxicity testing of nanomaterials (NMs) has been to simply adopt standardized aquatic toxicity tests. However, as these tests were primarily developed for soluble chemical, issues regarding the release of ions from metal-based NMs, particle agglomeration or sedimentation during the time of exposure are not considered when reporting the toxicity of NMs. So, these aspects may contribute to increase inconsistencies among studies. The aim of this work is to assess the toxicity of nine NMs (different nanoparticles of TiO2, ZnO, SiO2, BaSO4, bentonite and carbon nanotubes) towards rainbow trout following the OECD Test Guideline 203 by characterizing and monitoring the NM exposure during the test. The concentration of NMs was measured by UV-vis spectroscopy, inductively coupled plasma mass spectrometer or flame atomic absorption spectrometry depending on the type of NM. Dynamic Light Scattering was used for measuring the particle hydrodynamic size, but also for tracking particle stability over time. Fish mortalities were recorded at intervals of 24, 48, 72 and 96 h. Our findings might be used to develop protocols for ensuring homogeneous exposure which may strength reproducibility and interpretability and warrant read across and QSAR models.
Aknowledgments. This study was supported by the H2020 project NanoHarmony (GA nº 885931)


Keywords: nanomaterial; fish; protocol

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