Approved Abstracts

Investigating the potential bioaccumulation of CuO NMs in fish - influence of exposure route and shape on bioaccumulation potential



Author(s): National Institute for Agricultural and Food Research and Technology (INIA), Department of Environment and Agronomy, Madrid, Spain; National Institute for Agricultural and Food Research and Technology (INIA), Department of Environment and Agronomy, Madrid, Spain; Research Centre for Energy, Environment and Technology (CIEMAT), Division of Chemistry, Department of Technology, Madrid, Spain; Research Centre for Energy, Environment and Technology (CIEMAT), Division of Chemistry, Department of Technology, Madrid, Spain; National Institute for Agricultural and Food Research and Technology (INIA), Department of Environment and Agronomy, Madrid, Spain; Research Centre for Energy, Environment and Technology (CIEMAT), Division of Chemistry, Department of Technology, Madrid, Spain; National Institute for Agricultural and Food Research and Technology (INIA), Department of Environment and Agronomy, Madrid, Spain;
Presenter: Judit Kalman

Due to their wide use, copper oxide nanomaterials (CuO NMs) are likely to enter the aquatic environment, thus posing a possible threat to aquatic organisms, including fish. Despite this, the bioaccumulation potential of CuO NMs has scarcely been explored.
Dietary ingestion of NMs can be an important route of uptake for fish, thus in a first study we investigated the accumulation kinetics of a commercially available elliptical-shaped CuO NMs in rainbow trout (Oncorhynchus mykiss) following the OECD TG 305. Fish were fed diets containing 70 or 500 mg CuO NMs per kg body weight for 15 days and on control diets for a further 44 days. Cu concentrations were determined in the whole body and also in liver, gill, intestine, stomach, muscle, brain and fish carcasses. Results showed a significant increase in Cu concentration in the whole body of fish fed high dose diets during the uptake phase, although higher levels with respect to controls were also reached with the low dose. Fast initial elimination from fish bodies and specific tissues was observed during depuration, whereas in the liver elevated Cu concentrations were found at the end of depuration, showing that liver is a target organ for this NM. The low whole body biomagnification factor values suggest a low bioaccumulation potential for these elliptical-shaped CuO NMs.
The extent to which routes of exposure and NM properties such as shape influence accumulation kinetics is unknown. Therefore, in a second study we investigated the accumulation kinetics of a commercially available rod-shaped CuO NMs in O. mykiss via water and diet following the minimized aqueous and dietary bioaccumulation tests of the OECD TG 305. In the aqueous exposure, fish were exposed to 0.1 and 1 mg/L CuO NMs for 28 days, followed by 14 days of depuration. Cu concentrations were determined in the fish whole body to derive bioconcentration factors. For comparative purposes, in the dietary exposure study, the same exposure concentrations and time points for whole body and tissue sampling were selected as in the first study performed with the elliptical-shaped CuO NMs.
Results from these studies will address the importance and influence of the exposure route and NM shape in the accumulation kinetics of CuO NMs.
Acknowledgement: H2020 project GOV4NANO 814401 for its funding. Mona Connolly has received financing granted by the Community of Madrid (2018-T2/AMB-11392, Mode 2, Young Doctor Recruitment).


Keywords: bioaccumulation; rainbow trout; CuO NMs

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