Approved Abstracts

Bioaccumulation and metabolic response in the clam Scrobicularia plana exposed to cadmium and selenium combining an analytical metabolomic multiplatform with inorganic mass spectrometry

Author(s): Rodríguez-Moro G; Trombini C; Ramírez-Acosta S; Gómez-Ariza JL; Blasco J; García-Barrera T;
Presenter: Julian Blasco

Cadmium (Cd) is a common contaminant in the environment with a high persistent character and toxicity. However, metals are not found isolated in the environment and a wide number of synergistic and antagonistic interactions have been described in the literature [1,2]. One of the most known antagonistic element is selenium (Se), that balances the injurious effects of certain toxic metals and other pollutants [1,2]. Environmental metabolomics allows obtaining a global view of the metabolic fingerprint of biological systems exposed to pollutants, providing information about their interactions with living organisms [3]. In this study, Scrobicularia plana, which is considered a good bioindicator of environmental pollution, has been proposed to evaluate the metabolic changes induced by Cd and Se after 21 days of exposure. Clams were exposed to each element at two different concentrations (10 µg/L and 100 µg/L) separately and together to study the interactions between them (six groups and control). In addition, metals bioaccumulation in clam tissues was evaluated by inductively coupled plasma mass spectrometry (ICP-MS) throughout the experiment and after 7 days of depuration to study the possible elimination.
A metabolomic workflow was applied to the digestive gland of Scrobicularia plana based on the complementary use of both ultra-high performance liquid chromatography (UHPLC-QTOF-MS) and gas chromatography (GC-MS) to study the alterations in the metabolic pathways. The results show a greater number of altered metabolic pathways after the exposure to Cd, especially in the group exposed to 100 µg/L (~50 metabolites). However, a lower number of altered metabolic routes was observed after the intake of both indicating the possible beneficial antagonistic effect of Se against Cd toxicity. The bioaccumulation study reveals that there were not significant differences in the concentration of Se among the studied groups, but the bioaccumulation of Cd decreased when clams are co-exposed to Se.
[1] I. Messaoudi, J. El Heni, F. Hammouda, K. Saïd, A. Kerkeni. Biol. Trace Elem. Res. 130 (2009) 152-160.
[2] T. García-Barrera, J.L. Gómez-Ariza, M. González-Fernández, F. Moreno, M.A. García-Sevillano, V. Gómez-Jacinto. Anal. Bioanal. Chem. 403 (2012) 2237-2253
[2] J. G. Bundy, M. P. Davey, M. R. Viant. Metabolomics 5 (2008) 3–21.
This work was supported by the projects PG2018-096608-B-C21 and CTM2016-75908-R the Spanish Ministry of Economy and Competitiveness, Authors are grateful to FEDER (European Community) for financial support, Grant UNHU13-1E-1611. Rodríguez-Moro, G. thanks to Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020) y Fondo Social Europeo for postdoctoral grant (DOC_01115).

Keywords: clam; metals; metabolomic




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