Approved Abstracts

MOLECULAR DOCKING OF THE MALATHION INSECTICIDE WITH ZEBRAFISH CYTOSOLIC PHOSPHOLIPASE A2



Author(s): AGATHA MANZI DE CARLI; BRUNO PAES DE CARLI; AIRAM ROGGERO DOS SANTOS SILVA; LAILA LUCYANE FERREIRA DE MORAES; ISABELLY ANNUNCIATO; MARCOS ANTONIO DE OLIVEIRA; MARCOS HIKARI TOYAMA;
Presenter: Agatha Manzi De Carli

Malathion has been used for several decades to control insects of agricultural importance and to control disease vectors. In 2016, a report published by the World Health Organization concluded that organophosphorus such as malathion, glyphosate, dieldrin, and malaoxon should be restricted in several countries, but in Brazil, malathion is still widely used. The cytosolic calcium-dependent phospholipases A2 (cPLA2) are constitutive enzymes responsible for the remodeling and renewal the process of plasma membranes and for the synthesis of arachidonic acid, which is metabolized by cyclooxygenases (COX). Phylogenetic analysis shows that Danio rerio (zebrafish) has two major classes of PLA2s, the lipoprotein-associated PLA2s (PLA2-Lp) and another class that is the cytosolic calcium-dependent PLA2 (cPLA2α) and in both cases, their similarity to mammalian homologs is approximately 80%. Some studies show that cPLA2 α plays a crucial role in several cellular, physiological and pathological processes. In this study, we are investigating the action of malathion over zebrafish PLA2s. Through molecular homology and molecular docking approaches, we evaluate the molecular interactions of zebrafish cPLA2α with malathion. Our results suggest that the malathion has a high affinity with zebrafish cPLA2α (ΔG -5.4 kcal/mol), interacting strongly with Ala569 side chain through hydrogen bonds and also by several van der Waals and hydrophobic interactions indicating that malathion is a potent inhibitor of cytosolic PLA2α, which was indeed proven by in vitro assays. Because of the key role of cPLA2α in mediating the production of lipid mediators and its broad expression in tissues, it has been implicated in the regulation of homeostatic processes and in the pathogenesis of diseases in all organ systems. The importance of cPLA2α in the regulation of certain physiological systems, such as female reproduction and hemostasis, is more direct and can be attributed to specific types of lipid mediators. Studies in mammals concerning the decrease in cytosolic phospholipase A2 activity may include a wide range of impairment in autoimmunity, neurotoxicity due to lack of renewal of membrane phospholipids, renal activity, reproduction, and blood hemostasis. Thus, malathion on chronic exposure can trigger several long term adverse effects in fish.

Keywords: Organophosphorus; Toxicology; Phospholipase A2

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